International Journal of Animal Science and Technology

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Effect of Stainless Steel Tank Incubation System on Development of Ovine Embryos

Received: 15 September 2023    Accepted: 4 October 2023    Published: 14 October 2023
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Abstract

The utilization of separate culture chambers serves a dual purpose: it not only facilitates the creation of a hypoxic environment but also contributes to the establishment of a consistently stable local environment. This, in turn, mitigates environmental stress factors. The primary objective of this experiment was to assess the developmental potential of sheep embryos before their implantation. This assessment was conducted within the confines of 304 stainless steel culture chambers, offering a viable alternative for maintaining a stable in vitro embryo culture environment. The experiment involved a comparative analysis between the development outcomes of parthenogenetic and reconstituted sheep embryos cultured in both stainless steel tanks or MIC-101 incubators over a period of 7 days. Notably, the blastocyst rates for parthenogenetic embryos were 26.45% and 24.84% in stainless steel tanks and MIC-101 incubators, respectively, with no statistically significant difference (P>0.05) between them. Similarly, the blastocyst rates for reconstituted embryos stood at 16.07% and 16.84% (P>0.05) for stainless steel tanks and MIC-101 incubators, respectively. It is worth highlighting that the incubation system, comprising a 304 stainless steel tank, a standard gas mixture, and a thermostat, presents an in vitro culture system with the advantages of cost-effectiveness, environmental stability, and efficacy.

DOI 10.11648/j.ijast.20230703.12
Published in International Journal of Animal Science and Technology (Volume 7, Issue 3, September 2023)
Page(s) 43-47
Creative Commons

This is an Open Access article, distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution and reproduction in any medium or format, provided the original work is properly cited.

Copyright

Copyright © The Author(s), 2024. Published by Science Publishing Group

Keywords

Incubator, In Vitro Culture, Embryo, Sheep

References
[1] Swain JE. Decisions for the IVF laboratory: comparative analysis of embryo culture incubators. Reproductive biomedicine online vol. 28 (5): 535-547, 2014. DOI: 10.1016/j.rbmo.2014.01.004.
[2] Vajta G, Holm P, Greve T, Callesen H. The submarine incubation system, a new tool for in vitro embryo culture. A technique report. Theriogenology. 48 (8): 1379–1385, 1997. DOI: 10.1016/S0093-691X(97)00379-8.
[3] Vajta G, Bartels P, Joubert J, de la Rey M, Treadwell R, Callesen H. Production of a healthy calf by somatic cell nuclear transfer without micromanipulators and carbon dioxide incubators using the Handmade Cloning (HMC) and the Submarine Incubation System (SIS). Theriogenology. 62 (8): 1465-1472, 2004. DIO: 10.1016/j.theriogenology.2004.02.010.
[4] Speckhart SL, Wooldridge LK, Ealy AD. An updated protocol for in vitro bovine embryo production. STAR Protoc. 4 (1): 101924, 2022. DOI: 10.1016/j.xpro.2022.101924.
[5] Guo YH, Zhang YY, Wang LM, Tang H, Li YL, Zhou Ping. Program optimization before enucleation on ovine somatic cell nuclear transfer. Chinese Journal of Biotechnology. 33 (5): 730-742, 2017. DOI: 10.13345/j.cjb.160308.
[6] Vajta G, Rienzi L, Cobo A, Yovich J. Embryo culture: can we perform better than nature. Reproductive BioMedicine Online. 20 (4): 453-469, 2010. DOI: 10.1016/j.rbmo.2009.12.018.
[7] Nagao Y, Saeki K, Hoshi M, Kainuma H. Effects of oxygen concentration and oviductal epithelial tissue on the development of in vitro matured and fertilized bovine oocytes cultured in protein-free medium. Theriogenology. 41 (3): 681–687, 1994. DOI: 10.1016/0093-691x(94)90177-k.
[8] Karja NWK, Wongsrikeao P, Murakami M, Agung B, Fahrudin M, Nagai T, Otoi T. Effects of oxygen tension on the development and quality of porcine in vitro fertilized embryos. Theriogenology 62 (9): 1585-1595, 2004. DOI: 10.1016/j.theriogenology.2004.03.012.
[9] Leoni GG, Rosati I, Succu S, Bogliolo L, Bebbere D, Berlinguer F, Ledda S, Naitana S. A Low Oxygen Atmosphere during IVF Accelerates the Kinetic of Formation of In Vitro Produced Ovine Blastocyst. Reproduction in Domestic Animals. 42 (3): 299-304, 2007. DOI: 10.1111/j.1439-0531.2006.00783.x.
[10] Lee SC, Seo HC, Lee J, Jun JH, Choi KW. Effects of dynamic oxygen concentrations on the development of mouse pre- and peri-implantation embryos using a double-channel gas supply incubator system. Clin Exp Reprod Med. 46 (4): 189-196, 2019. DOI: 10.5653/cerm.2019.00514.
[11] Li M, Xue X, Shi J. Ultralow Oxygen Tension (2%) Is Beneficial for Blastocyst Formation of In Vitro Human Low-Quality Embryo Culture. Biomed Res Int. 9603185, 2022. DOI: 10.1155/2022/9603185.
[12] Meintjes M, Chantilis SJ, Douglas JD, Rodriguez AJ, Guerami AR, Bookout DM, Barnett BD, Madden JD. A controlled randomized trial evaluating the effect of lowered incubator oxygen tension on live births in a predominantly blastocyst transfer program. Human Reproduction. 24 (2): 300–307, 2009. DOI: 10.1093/humrep/den368.
[13] Du Y, Lin L, Schmidt M, Bøgh IB, Kragh PM, Sørensen CB, Li J, Purup S, Pribenszky C, Molnár M, Kuwayama M, Zhang X, Yang H, Bolund L, Vajta G. High hydrostatic pressure treatment of porcine oocytes before handmade cloning improves developmental competence and cryosurvival. Cloning Stem Cells. 10 (3): 325–330, 2008. DOI: 10.1089/clo.2007.0089.
[14] Knudtson JF, Robinson RD, Sparks AE, Hill MJ, Chang TA, Van Voorhis BJ. Common practices among consistently high-performing in vitro fertilization programs in the United States: 10-year update. Fertil Steril. 117 (1): 42-50, 2022. DOI: 10.1016/j.fertnstert.2021.09.010.
[15] de Lima CB, Dos Santos ÉC, Ispada J, Fontes PK, Nogueira MFG, Dos Santos CMD, Milazzotto MP. The dynamics between in vitro culture and metabolism: embryonic adaptation to environmental changes. Sci Rep. 10 (1): 15672, 2020. DOI: 10.1038/s41598-020-72221-1.
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  • APA Style

    Feng Guangyu, Xiang Chunhe, Wang Limin, Zhou Ping, Pi Wenhui. (2023). Effect of Stainless Steel Tank Incubation System on Development of Ovine Embryos. International Journal of Animal Science and Technology, 7(3), 43-47. https://doi.org/10.11648/j.ijast.20230703.12

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    ACS Style

    Feng Guangyu; Xiang Chunhe; Wang Limin; Zhou Ping; Pi Wenhui. Effect of Stainless Steel Tank Incubation System on Development of Ovine Embryos. Int. J. Anim. Sci. Technol. 2023, 7(3), 43-47. doi: 10.11648/j.ijast.20230703.12

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    AMA Style

    Feng Guangyu, Xiang Chunhe, Wang Limin, Zhou Ping, Pi Wenhui. Effect of Stainless Steel Tank Incubation System on Development of Ovine Embryos. Int J Anim Sci Technol. 2023;7(3):43-47. doi: 10.11648/j.ijast.20230703.12

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  • @article{10.11648/j.ijast.20230703.12,
      author = {Feng Guangyu and Xiang Chunhe and Wang Limin and Zhou Ping and Pi Wenhui},
      title = {Effect of Stainless Steel Tank Incubation System on Development of Ovine Embryos},
      journal = {International Journal of Animal Science and Technology},
      volume = {7},
      number = {3},
      pages = {43-47},
      doi = {10.11648/j.ijast.20230703.12},
      url = {https://doi.org/10.11648/j.ijast.20230703.12},
      eprint = {https://article.sciencepublishinggroup.com/pdf/10.11648.j.ijast.20230703.12},
      abstract = {The utilization of separate culture chambers serves a dual purpose: it not only facilitates the creation of a hypoxic environment but also contributes to the establishment of a consistently stable local environment. This, in turn, mitigates environmental stress factors. The primary objective of this experiment was to assess the developmental potential of sheep embryos before their implantation. This assessment was conducted within the confines of 304 stainless steel culture chambers, offering a viable alternative for maintaining a stable in vitro embryo culture environment. The experiment involved a comparative analysis between the development outcomes of parthenogenetic and reconstituted sheep embryos cultured in both stainless steel tanks or MIC-101 incubators over a period of 7 days. Notably, the blastocyst rates for parthenogenetic embryos were 26.45% and 24.84% in stainless steel tanks and MIC-101 incubators, respectively, with no statistically significant difference (P>0.05) between them. Similarly, the blastocyst rates for reconstituted embryos stood at 16.07% and 16.84% (P>0.05) for stainless steel tanks and MIC-101 incubators, respectively. It is worth highlighting that the incubation system, comprising a 304 stainless steel tank, a standard gas mixture, and a thermostat, presents an in vitro culture system with the advantages of cost-effectiveness, environmental stability, and efficacy.},
     year = {2023}
    }
    

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  • TY  - JOUR
    T1  - Effect of Stainless Steel Tank Incubation System on Development of Ovine Embryos
    AU  - Feng Guangyu
    AU  - Xiang Chunhe
    AU  - Wang Limin
    AU  - Zhou Ping
    AU  - Pi Wenhui
    Y1  - 2023/10/14
    PY  - 2023
    N1  - https://doi.org/10.11648/j.ijast.20230703.12
    DO  - 10.11648/j.ijast.20230703.12
    T2  - International Journal of Animal Science and Technology
    JF  - International Journal of Animal Science and Technology
    JO  - International Journal of Animal Science and Technology
    SP  - 43
    EP  - 47
    PB  - Science Publishing Group
    SN  - 2640-1312
    UR  - https://doi.org/10.11648/j.ijast.20230703.12
    AB  - The utilization of separate culture chambers serves a dual purpose: it not only facilitates the creation of a hypoxic environment but also contributes to the establishment of a consistently stable local environment. This, in turn, mitigates environmental stress factors. The primary objective of this experiment was to assess the developmental potential of sheep embryos before their implantation. This assessment was conducted within the confines of 304 stainless steel culture chambers, offering a viable alternative for maintaining a stable in vitro embryo culture environment. The experiment involved a comparative analysis between the development outcomes of parthenogenetic and reconstituted sheep embryos cultured in both stainless steel tanks or MIC-101 incubators over a period of 7 days. Notably, the blastocyst rates for parthenogenetic embryos were 26.45% and 24.84% in stainless steel tanks and MIC-101 incubators, respectively, with no statistically significant difference (P>0.05) between them. Similarly, the blastocyst rates for reconstituted embryos stood at 16.07% and 16.84% (P>0.05) for stainless steel tanks and MIC-101 incubators, respectively. It is worth highlighting that the incubation system, comprising a 304 stainless steel tank, a standard gas mixture, and a thermostat, presents an in vitro culture system with the advantages of cost-effectiveness, environmental stability, and efficacy.
    VL  - 7
    IS  - 3
    ER  - 

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Author Information
  • College of Animal Science and Technology, Shihezi University, Shihezi, China

  • College of Animal Science and Technology, Shihezi University, Shihezi, China

  • State Key Laboratory of Sheep Genetic Improvement and Healthy Breeding, Xinjiang Academy of Agricultural and Reclamation Sciences, Shihezi, China; Institute of Animal Husbandry and Veterinary Medicine, Xinjiang Academy of Agricultural and Reclamation Sciences, Shihezi, China

  • State Key Laboratory of Sheep Genetic Improvement and Healthy Breeding, Xinjiang Academy of Agricultural and Reclamation Sciences, Shihezi, China; Institute of Animal Husbandry and Veterinary Medicine, Xinjiang Academy of Agricultural and Reclamation Sciences, Shihezi, China

  • College of Animal Science and Technology, Shihezi University, Shihezi, China

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