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1College of Animal Science and Technology, Shihezi University, Shihezi, China
2State Key Laboratory of Sheep Genetic Improvement and Healthy Breeding, Xinjiang Academy of Agricultural and Reclamation Sciences, Shihezi, China
3Institute of Animal Husbandry and Veterinary Medicine, Xinjiang Academy of Agricultural and Reclamation Sciences, Shihezi, China
The utilization of separate culture chambers serves a dual purpose: it not only facilitates the creation of a hypoxic environment but also contributes to the establishment of a consistently stable local environment. This, in turn, mitigates environmental stress factors. The primary objective of this experiment was to assess the developmental potential of sheep embryos before their implantation. This assessment was conducted within the confines of 304 stainless steel culture chambers, offering a viable alternative for maintaining a stable in vitro embryo culture environment. The experiment involved a comparative analysis between the development outcomes of parthenogenetic and reconstituted sheep embryos cultured in both stainless steel tanks or MIC-101 incubators over a period of 7 days. Notably, the blastocyst rates for parthenogenetic embryos were 26.45% and 24.84% in stainless steel tanks and MIC-101 incubators, respectively, with no statistically significant difference (P>0.05) between them. Similarly, the blastocyst rates for reconstituted embryos stood at 16.07% and 16.84% (P>0.05) for stainless steel tanks and MIC-101 incubators, respectively. It is worth highlighting that the incubation system, comprising a 304 stainless steel tank, a standard gas mixture, and a thermostat, presents an in vitro culture system with the advantages of cost-effectiveness, environmental stability, and efficacy.
Incubator, In Vitro Culture, Embryo, Sheep
Feng Guangyu, Xiang Chunhe, Wang Limin, Zhou Ping, Pi Wenhui. (2023). Effect of Stainless Steel Tank Incubation System on Development of Ovine Embryos. International Journal of Animal Science and Technology, 7(3), 43-47. https://doi.org/10.11648/j.ijast.20230703.12
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